Evaluación del crecimiento de bacterias endófitas halotolerantes de manglar para su uso potencial en la producción de un bionoculante
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Fecha
2023
Autores
Serna De la Hoz, Arnaldo José
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Ediciones Universidad Simón Bolívar
Facultad de Ciencias Básicas y Biomédicas
Facultad de Ciencias Básicas y Biomédicas
Resumen
Las bacterias endófitas halotolerantes que se encuentran en los manglares y que poseen características propicias para el estímulo del crecimiento vegetal se presentan como una opción viable para su empleo como bioinoculantes, especialmente en situaciones donde las plantas enfrentan condiciones adversas de estrés abiótico, como la sequía y la salinidad en el suelo. La selección de la cepa bacteriana adecuada que cumpla con los requisitos mencionados conlleva la necesidad de calcular y analizar los parámetros cinéticos asociados al crecimiento en diferentes medios de cultivo. Además, es fundamental evaluar la formación de biopelícula, ya que su presencia podría plantear desafíos en el proceso de escalado para la producción de un bioinoculante que involucrará la encapsulación de bacterias mediante el empleo del alginato de sodio. El objetivo del trabajo fue evaluar el crecimiento de bacterias halotolerantes para su uso potencial en la producción de un bioinoculante; para ello se estandarizaron preinóculos de 9 cepas endófitas aisladas de manglares en dos medios de cultivos: caldo LB y caldo TSB, el crecimiento de los inóculos y la producción de biopelículas se evaluó con un lector de microplacas midiendo densidad óptica a 600 nm durante 24 a 48 h. La cepa C7 es la de mejor crecimiento con μ: 0.16 h-1 y Td: 4.42 h en LB, y μ: 0.12 h-1 y Td: 5.71 h en TSB. No obstante, la cepa C3 presentó el mayor crecimiento con μ: 0.19 h-1, Td de 3.56 h y formación de biopelícula en LB y una μ de 0.23 h-1 y un Td de 2.90 h en TSB, presentando gráficas atípicas en ambos medios. Se infiere que la producción de biomasa en las cepas evaluadas está influenciada por el medio de cultivo, la formación de biopelícula y el tiempo de fase exponencial. Se pretende continuar el estudio evaluando el crecimiento de la cepa C7 en Erlenmeyer por 24 h, midiendo densidad óptica y haciendo recuento de viables por la técnica de microdiluciones y microgota cada 60 minutos
The halotolerant endophytic bacteria found in mangroves, exhibiting favorable characteristics for stimulating plant growth, emerge as a viable option for use as bioinoculants, especially in situations where plants face adverse abiotic stress conditions such as drought and soil salinity. Selecting the appropriate bacterial strain meeting these requirements involves the necessity to calculate and analyze the kinetic parameters associated with growth in different culture media. Additionally, it is crucial to assess biofilm formation, as its presence may pose challenges in the scaling-up process for bioinoculant production, involving bacterial encapsulation using sodium alginate. The objective of this study was to evaluate the growth of halotolerant bacteria for their potential use in bioinoculant production. To do this, pre-inoculants of 9 endophytic strains isolated from mangroves were standardized in two culture media: LB broth and TSB broth. Inoculum growth and biofilm production were assessed using a microplate reader by measuring optical density at 600 nm over 24 to 48 hours. Strain 7 exhibited the best growth with μ: 0.16 h-1 and dT: 4.42 h in LB, and μ: 0.12 h-1 and dT: 5.71 h in TSB. However, strain 3 showed the highest growth with μ: 0.19 h-1, dT of 3.56 h, and biofilm formation in LB, and a μ of 0.24 h-1 and a dT of 2.90 h in TSB, displaying atypical growth curves in both media. It is inferred that biomass production in the evaluated strains is influenced by the culture medium, biofilm formation, and the time of exponential phase. The study intends to continue by evaluating the growth of strain 7 in Erlenmeyer for 24 hours, measuring optical density, and performing viable counts using the microdilution and microdilution methods every 60 minutes.
The halotolerant endophytic bacteria found in mangroves, exhibiting favorable characteristics for stimulating plant growth, emerge as a viable option for use as bioinoculants, especially in situations where plants face adverse abiotic stress conditions such as drought and soil salinity. Selecting the appropriate bacterial strain meeting these requirements involves the necessity to calculate and analyze the kinetic parameters associated with growth in different culture media. Additionally, it is crucial to assess biofilm formation, as its presence may pose challenges in the scaling-up process for bioinoculant production, involving bacterial encapsulation using sodium alginate. The objective of this study was to evaluate the growth of halotolerant bacteria for their potential use in bioinoculant production. To do this, pre-inoculants of 9 endophytic strains isolated from mangroves were standardized in two culture media: LB broth and TSB broth. Inoculum growth and biofilm production were assessed using a microplate reader by measuring optical density at 600 nm over 24 to 48 hours. Strain 7 exhibited the best growth with μ: 0.16 h-1 and dT: 4.42 h in LB, and μ: 0.12 h-1 and dT: 5.71 h in TSB. However, strain 3 showed the highest growth with μ: 0.19 h-1, dT of 3.56 h, and biofilm formation in LB, and a μ of 0.24 h-1 and a dT of 2.90 h in TSB, displaying atypical growth curves in both media. It is inferred that biomass production in the evaluated strains is influenced by the culture medium, biofilm formation, and the time of exponential phase. The study intends to continue by evaluating the growth of strain 7 in Erlenmeyer for 24 hours, measuring optical density, and performing viable counts using the microdilution and microdilution methods every 60 minutes.
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Palabras clave
Bacteria endófita, Cinética del crecimiento, Bioinoculante, Biopelícula, Endophytic bacteria, Growth kinetics, Bioinoculant, Biofilm
