Estudio de bacteriófagos como modelo de terapia antimicrobiana
Archivos
Fecha
2014
Autores
López Arévalo, Mary Isabel
Márquez Ariza, Carlos Mario
Marenco Salazar, Juan David
Rojas Garrido, Roxana Milena
Título de la revista
ISSN de la revista
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Editor
Ediciones Universidad Simón Bolívar
Facultad de Ciencias de la Salud
Facultad de Ciencias de la Salud
Resumen
La resistencia bacteriana es uno de los mayores problemas en salud pública evidenciado por el aumento considerable en la tasa de tratamientos fallidos en enfermedades infecciosas; esto como consecuencia de la alta resistencia a los agentes antimicrobianos. Los bacteriófagos son virus que infectan a las bacterias y podrían postularse como una terapia alternativa para dichas enfermedades, ya que son seguros, específicos, no infectan a la especie humana y son capaces de adaptarse a los cambios que la bacteria genere y así matarlas. Metodología: Se obtuvieron muestras del rio Magdalena y se sometió a técnicas para aislamiento de fagos, de cepas resistentes de E, coli y klebsiella pneumoniae para el enriquecimiento inicial de bacteriófagos de rio; luego se aisló el fago y se contaron las unidades formadoras de placas.
Bacterial resistance is a major public health problem today as evidenced by the significant increase in the rate of failure in infectious disease treatments; this is due to the high resistance to antimicrobial agents. Bacteriophages are visures that infect bacteria and could stand as an alternative therapy for these infections, because they are safe, specific, do not infect the human species and are able to adapt to changes by their host bacteria. Water samples were obtained from the Magdalena River, and was subject to several molecular biology techniques for phage enrichment. Multiresistant E. coli and klebsiella pneumoniae was used in this study and was challenged with river water fracttions for phage isolation.
Bacterial resistance is a major public health problem today as evidenced by the significant increase in the rate of failure in infectious disease treatments; this is due to the high resistance to antimicrobial agents. Bacteriophages are visures that infect bacteria and could stand as an alternative therapy for these infections, because they are safe, specific, do not infect the human species and are able to adapt to changes by their host bacteria. Water samples were obtained from the Magdalena River, and was subject to several molecular biology techniques for phage enrichment. Multiresistant E. coli and klebsiella pneumoniae was used in this study and was challenged with river water fracttions for phage isolation.
Descripción
Palabras clave
Bacteriófago, Aislamiento, Cultivo, Terapia antimicrobiana, Unidades formadoras de placas, Klebsiella pneumonie, Bacteriophage, Isolation, Culture, Antimicrobial therapy, Plaque forming units, Kllebsiella pnuemoniae
