Caracterización filogenética de Carapa guianensis en el bosque húmedo tropical de Colombia
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Fecha
2021
Autores
Flórez Oviedo, Laura Vanessa
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Ediciones Universidad Simón Bolivar
Facultad Ciencias Básicas y Biomédicas
Facultad Ciencias Básicas y Biomédicas
Resumen
Carapa Guianensis pertenece a la familia Meliaceae. Esta especie habita en los bosques húmedos de Colombia. La especie se destaca por poseer maderas de calidad, que han sido ampliamente explotadas por ser una de las más utilizadas en la construcción, decoración, entre otros; además, el aceite de las semillas se usa para hacer jabones y en el área cosmetológica. Tomando como problemática del estudio categorizar el estado de conservación en el que se encuentra Carapa guianensis. El objetivo principal del trabajo fue realizar un análisis filogenético de aislados de Carapa guianensis, teniendo en cuenta los marcadores moleculares como el gen cloroplastídial (rbcL), maturase K (mtK) y regiones espaciadoras intergénicas como trnH-psbA y trnL-trnF. Inicialmente se buscaron la secuencia de los marcadores seleccionados en la base de datos del GenBank del NCBI, se editaron y alinearán usando el programa BioEdit. Luego se construyeron dendrogramas empleando el programa MEGA X usando métodos de agrupamiento por distancia (Neighbour-Joining y UPGMA) y por filogenia (Máxima Verosimilitud y Máxima Parsimonia). Finalmente, se estandarizaron los procedimientos de laboratorio que permitirán determinar el estado de conservación en el que se encuentra la especie.
Carapa guianensis belongs to the Meliaceae family. This species inhabits the humid forests of Colombia. The species stands out for its high quality wood, which has been widely exploited for being one of the most used in construction, decoration, among others; in addition, the oil from the seeds is used to make soaps and in the cosmetology area. The problem of the study was to categorize the conservation status of Carapa guianensis. The main objective of the work was to perform a phylogenetic analysis of Carapa guianensis isolates, taking into account molecular markers such as the chloroplastic gene (rbcL), maturase K (mtK) and intergenic spacer regions such as trnH-psbA and trnL-trnF. Initially, the sequence of the selected markers was searched in the NCBI GenBank database, edited and aligned using the BioEdit program. Then, dendrograms were constructed using the MEGA X program using clustering methods by distance (Neighbour-Joining and UPGMA) and by phylogeny (Maximum Likelihood and Maximum Parsimony). Finally, laboratory procedures were standardized to determine the conservation status of the species.
Carapa guianensis belongs to the Meliaceae family. This species inhabits the humid forests of Colombia. The species stands out for its high quality wood, which has been widely exploited for being one of the most used in construction, decoration, among others; in addition, the oil from the seeds is used to make soaps and in the cosmetology area. The problem of the study was to categorize the conservation status of Carapa guianensis. The main objective of the work was to perform a phylogenetic analysis of Carapa guianensis isolates, taking into account molecular markers such as the chloroplastic gene (rbcL), maturase K (mtK) and intergenic spacer regions such as trnH-psbA and trnL-trnF. Initially, the sequence of the selected markers was searched in the NCBI GenBank database, edited and aligned using the BioEdit program. Then, dendrograms were constructed using the MEGA X program using clustering methods by distance (Neighbour-Joining and UPGMA) and by phylogeny (Maximum Likelihood and Maximum Parsimony). Finally, laboratory procedures were standardized to determine the conservation status of the species.
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Método máxima verosimilitud, MtK, Parsimonia, rbcL, Secuencia de cloroplasto, trnH-psbA, trnL-trnF, Likelihood method, MtK, Parsimony, Chloroplast sequence, rbcL, trnH-psbA, trnL-trnF