Diversificación genética en poblaciones de Panstrongylus Geniculatus (Latreille, 1811) (Reduviidae: triatominae) de Colombia y Venezuela
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Fecha
2021
Autores
Londoño Alvarez, Juan Carlos
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Ediciones Universidad Simón Bolívar
Facultad de Ciencias Básicas y Biomédicas
Facultad de Ciencias Básicas y Biomédicas
Resumen
Objetivo: Determinar la diversificación genética en poblaciones de Panstrongylus
geniculatus de Colombia y Venezuela.
Metodología: se analizaron 202 secuencias del gen Cyt-b de P. geniculatus de Colombia y
Venezuela, a fin de calcular índices de diversidad genética, de estructura y flujo génico,
evaluar hipótesis de evolución neutral y realizar inferencias filogenéticas.
Resultados: el conglomerado de P. geniculatus de Venezuela, presentó una diversidad
haplotípica inferior (Hd = 0,73) respecto al de Colombia (0,93). Los pares de poblaciones
Casanare-Leticia, Sucre-Santanderes y Libertador-La Guaira no presentaron estructuración
genética (FST 0,05 y Nm > 1). Se registraron desviaciones de la hipótesis de evolución neutral
en las poblaciones del Meta en Colombia (D* = 1,44347) y La Guaira en Venezuela (D = -
1,67405; D* = 1,82716). El análisis filogenético mostró tres grandes clados, uno exclusivo
para Colombia, uno para Venezuela y uno mixto, la red de haplotipos permitió observar
cuatro haplogrupos principales.
Conclusiones: El haplotipo ancestral de las poblaciones de P. geniculatus pudo estar
distribuido en un corredor conformado por los departamentos de Sucre, Bolívar y Santander
en Colombia
Objective: To determine genetic diversification in of Panstrongylus geniculatus popilations from Colombia and Venezuela. Methodology: 202 Cyt-b gene sequences of P. geniculatus from Colombia and Venezuela were analyzed in order to calculate genetic diversity, structure and gene flow indexes, make hypotheses of neutral evolution test and make phylogenetic inferences. Results: The P. geniculatus cluster from Venezuela had a lower haplotypic diversity (Hd = 0.73) than Colombia cluster (0.93). The Casanare-Leticia, Sucre-Santanderes and LibertadorLa Guaira population pairs did not show genetic structuring (FST 0.05 and Nm > 1). Deviations from the hypothesis of neutral evolution were recorded in the populations of Meta in Colombia (D* = 1.44347) and La Guaira in Venezuela (D = -1.67405; D* = 1.82716). Phylogenetic analysis showed three major clades, one exclusive for Colombia, one for Venezuela and one mixed, the haplotype network allowed observing four major haplogroups. Conclusions: The ancestral haplotype of P. geniculatus populations could be distributed in a corridor conformed by the departments of Sucre, Bolivar and Santander in Colombia.
Objective: To determine genetic diversification in of Panstrongylus geniculatus popilations from Colombia and Venezuela. Methodology: 202 Cyt-b gene sequences of P. geniculatus from Colombia and Venezuela were analyzed in order to calculate genetic diversity, structure and gene flow indexes, make hypotheses of neutral evolution test and make phylogenetic inferences. Results: The P. geniculatus cluster from Venezuela had a lower haplotypic diversity (Hd = 0.73) than Colombia cluster (0.93). The Casanare-Leticia, Sucre-Santanderes and LibertadorLa Guaira population pairs did not show genetic structuring (FST 0.05 and Nm > 1). Deviations from the hypothesis of neutral evolution were recorded in the populations of Meta in Colombia (D* = 1.44347) and La Guaira in Venezuela (D = -1.67405; D* = 1.82716). Phylogenetic analysis showed three major clades, one exclusive for Colombia, one for Venezuela and one mixed, the haplotype network allowed observing four major haplogroups. Conclusions: The ancestral haplotype of P. geniculatus populations could be distributed in a corridor conformed by the departments of Sucre, Bolivar and Santander in Colombia.
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Palabras clave
P. geniculatus, Diversidad genética, Estructura poblacional, Filogenia, Filogeografía, P. geniculatus, Genetic diversity, Population structure, Phylogeny, Phylogeography, Colombia, Venezuela