Evaluación de marcadores moleculares para el estudio poblacional de Lecythis minor Jacq. (Lecythidaceae)
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Fecha
2020
Autores
Navas Ramírez, Alfredo Miguel
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Ediciones Universidad Simón Bolívar
Facultad de Ciencias Básicas y Biomédicas
Facultad de Ciencias Básicas y Biomédicas
Resumen
El bosque seco tropical (Bs-T) es un ecosistema importante para distintos grupos de
organismos debido a su alta diversidad florística. Dentro de la amplia diversidad de especies
vegetales del Bs-T se encuentra Lecythis minor, un árbol distribuido en el norte de Suramérica en
Venezuela y Colombia. Esta especie poco estudiada está empezando a ser utilizada en procesos de
reforestación y posee un potencial económico, debido a la comercialización del aceite rico en
selenio orgánico que se encuentra en sus semillas. Por tanto, es necesario identificar marcadores
moleculares que puedan ser utilizados para el estudio de la genética poblacional de L. minor. En este
sentido, se planteó evaluar marcadores moleculares para el estudio poblacional de L. minor. Para
ello se evaluó un set de marcadores ISSR y SSR, probando la transferibilidad de estos en L. minor.
Teniendo como resultado 5 marcadores ISSR amplificados, los cuales generaron un total de 31
bandas, de las cuales 25 fueron polimórficas; mientras que de los 10 SSR probados se amplificaron
7, obteniendo un set de marcadores con un promedio de PIC de 0,447. Se obtuvo un set de 5
marcadores ISSR y 5 marcadores SSR informativos transferidos a la especie, convirtiéndose en una
herramienta para futuros estudios en genética poblacional de L. minor.
The tropical dry forest (Bs-T) is an important ecosystem for different groups of organisms due to its high floristic diversity. Within the wide diversity of plant species of Bs-T is Lecythis minor, a tree distributed in the north of South America in Venezuela and Colombia. This little-studied species is beginning to be used in reforestation processes and has economic potential, due to the commercialization of the oil rich in organic selenium found in its seeds. Therefore, it is necessary to identify molecular markers that can be used to study the population genetics of L. minor. In this sense, it was proposed to evaluate molecular markers for the population study of L. minor. For this, a set of ISSR and SSR markers was evaluated, testing their transferability in L. minor. Resulting in 5 amplified ISSR markers, which generated a total of 31 bands, of which 25 were polymorphic; While of the 10 SSRs tested, 7 were amplified, obtaining a set of markers with an average ICP of 0.447. A set of 5 ISSR markers and 5 informative SSR markers transferred to the species were obtained, becoming a tool for future studies in population genetics of L. minor.
The tropical dry forest (Bs-T) is an important ecosystem for different groups of organisms due to its high floristic diversity. Within the wide diversity of plant species of Bs-T is Lecythis minor, a tree distributed in the north of South America in Venezuela and Colombia. This little-studied species is beginning to be used in reforestation processes and has economic potential, due to the commercialization of the oil rich in organic selenium found in its seeds. Therefore, it is necessary to identify molecular markers that can be used to study the population genetics of L. minor. In this sense, it was proposed to evaluate molecular markers for the population study of L. minor. For this, a set of ISSR and SSR markers was evaluated, testing their transferability in L. minor. Resulting in 5 amplified ISSR markers, which generated a total of 31 bands, of which 25 were polymorphic; While of the 10 SSRs tested, 7 were amplified, obtaining a set of markers with an average ICP of 0.447. A set of 5 ISSR markers and 5 informative SSR markers transferred to the species were obtained, becoming a tool for future studies in population genetics of L. minor.
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Palabras clave
ISSR, SSR, Diversidad genética, Variabilidad genética, Genetic diversity, Genetic variability