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dc.contributor.authorNavas Ramírez, Alfredo Miguel
dc.date.accessioned2020-12-09T16:50:09Z
dc.date.available2020-12-09T16:50:09Z
dc.date.issued2020
dc.identifier.urihttps://hdl.handle.net/20.500.12442/6857
dc.description.abstractEl bosque seco tropical (Bs-T) es un ecosistema importante para distintos grupos de organismos debido a su alta diversidad florística. Dentro de la amplia diversidad de especies vegetales del Bs-T se encuentra Lecythis minor, un árbol distribuido en el norte de Suramérica en Venezuela y Colombia. Esta especie poco estudiada está empezando a ser utilizada en procesos de reforestación y posee un potencial económico, debido a la comercialización del aceite rico en selenio orgánico que se encuentra en sus semillas. Por tanto, es necesario identificar marcadores moleculares que puedan ser utilizados para el estudio de la genética poblacional de L. minor. En este sentido, se planteó evaluar marcadores moleculares para el estudio poblacional de L. minor. Para ello se evaluó un set de marcadores ISSR y SSR, probando la transferibilidad de estos en L. minor. Teniendo como resultado 5 marcadores ISSR amplificados, los cuales generaron un total de 31 bandas, de las cuales 25 fueron polimórficas; mientras que de los 10 SSR probados se amplificaron 7, obteniendo un set de marcadores con un promedio de PIC de 0,447. Se obtuvo un set de 5 marcadores ISSR y 5 marcadores SSR informativos transferidos a la especie, convirtiéndose en una herramienta para futuros estudios en genética poblacional de L. minor.spa
dc.description.abstractThe tropical dry forest (Bs-T) is an important ecosystem for different groups of organisms due to its high floristic diversity. Within the wide diversity of plant species of Bs-T is Lecythis minor, a tree distributed in the north of South America in Venezuela and Colombia. This little-studied species is beginning to be used in reforestation processes and has economic potential, due to the commercialization of the oil rich in organic selenium found in its seeds. Therefore, it is necessary to identify molecular markers that can be used to study the population genetics of L. minor. In this sense, it was proposed to evaluate molecular markers for the population study of L. minor. For this, a set of ISSR and SSR markers was evaluated, testing their transferability in L. minor. Resulting in 5 amplified ISSR markers, which generated a total of 31 bands, of which 25 were polymorphic; While of the 10 SSRs tested, 7 were amplified, obtaining a set of markers with an average ICP of 0.447. A set of 5 ISSR markers and 5 informative SSR markers transferred to the species were obtained, becoming a tool for future studies in population genetics of L. minor.eng
dc.format.mimetypepdfspa
dc.language.isospaspa
dc.publisherEdiciones Universidad Simón Bolívarspa
dc.publisherFacultad de Ciencias Básicas y Biomédicasspa
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectISSRspa
dc.subjectSSRspa
dc.subjectDiversidad genéticaspa
dc.subjectVariabilidad genéticaspa
dc.subjectGenetic diversityeng
dc.subjectGenetic variabilityeng
dc.titleEvaluación de marcadores moleculares para el estudio poblacional de Lecythis minor Jacq. (Lecythidaceae)spa
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sb.sedeSede Barranquillaspa
sb.programaMaestría en Genéticaspa
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccessspa
datacite.rightshttp://purl.org/coar/access_right/c_16ecspa
oaire.versioninfo:eu-repo/semantics/acceptedVersionspa
dc.type.driverinfo:eu-repo/semantics/masterThesiseng
dc.type.spaTrabajo de grado másterspa


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