Hepatic and neurobiological effects of foetal and breastfeeding and adulthood exposure to methylmercury in Wistar rats
No hay miniatura disponible
Fecha
2019
Autores
da Rosa-Silva, Helen Tais
Castro Panzenhagen, Alana
Schmidtt, Victória
Alves Teixeira, Alexsander
Espitia-Pérez, Pedro
de Oliveira Franco, Álvaro
Mingori, Moara
Torres-Ávila, José F.
Schnorr, Carlos Eduardo
Silva Hermann, Paolla Rissi
Título de la revista
ISSN de la revista
Título del volumen
Editor
ELSevier
Resumen
Methylmercury (MeHg) is an organic bioaccumulated mercury derivative that strongly affects the
environment and represents a public health problem primarily to riparian communities in South
America. Our objective was to investigate the hepatic and neurological effects of MeHg exposure during
the phases foetal and breast-feeding and adult in Wistar rats. Wistar rats (n = 10) were divided into 3
groups. Control group received mineral oil; The simple exposure (SE) group was exposed only in
adulthood (0.5 mg/kg/day); and double exposure (DE) was pre-exposed to MeHg 0.5 mg/kg/day during
pregnancy and breastfeeding (±40 days) and re-exposed to MeHg for 45 days from day 100. After, we
evaluated possible abnormalities. Behavioral and biochemical parameters in liver and occipital cortex
(CO), markers of liver injury, redox and AKT/GSK3b/mTOR signaling pathway. Our results showed that
both groups treated with MeHg presented significant alterations, such as decreased locomotion and
exploration and impaired visuospatial perception. The rats exposed to MeHg showed severe liver damage and increased hepatic glycogen concentration. The MeHg groups showed significant impairment in redox
balance and oxidative damage to liver macromolecules and CO. MeHg upregulated the AKT/GSK3b/mTOR
pathway and the phosphorylated form of the Tau protein. In addition, we found a reduction in NeuN and
GFAP immunocontent. These results represent the first approach to the hepatotoxic and neural effects of
foetal and adult MeHg exposure.
Descripción
Palabras clave
Methylmercury, Double exposure, Double exposure, Hepatotoxicity, Neurotoxicity