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dc.contributor.authorKalempa Demeu, Lara Maria
dc.contributor.authorJahn Soares, Rodrigo
dc.contributor.authorSevero Miranda, Juliana
dc.contributor.authorPacheco-Lugo, Lisandro A.
dc.contributor.authorGonçalves Oliveira, Kelin
dc.contributor.authorCortez Plaza, Cristian Andrés
dc.contributor.authorBilliald, Philippe
dc.contributor.authorFerreira de Moura, Juliana
dc.contributor.authorYoshida, Nobuko
dc.contributor.authorMagalhães Alvarenga, Larissa
dc.contributor.authorDuarte DaRocha, Wanderson
dc.date.accessioned2019-10-17T14:51:31Z
dc.date.available2019-10-17T14:51:31Z
dc.date.issued2019-10
dc.identifier.urihttps://hdl.handle.net/20.500.12442/4168
dc.description.abstractTrypanosoma cruzi is a flagellate protozoan pathogen that causes Chagas disease. Currently there is no preventive treatment and the efficiency of the two drugs available is limited to the acute phase. Therefore, there is an unmet need for innovative tools to block transmission in endemic areas. In this study, we engineered a novel recombinant molecule able to adhere to the T. cruzi surface, termed scFv-10D8, that consists of a single-chain variable fragment (scFv) derived from mAb-10D8 that targets gp35/50. The synthetic gene encoding scFv-10D8 was cloned and fused to a 6×His tag and expressed in a prokaryotic expression system. Total periplasmic or 6xHis tag affinity-purified fractions of scFv-10D8 retained the capacity to bind to gp35/50, as shown by Western blot analyses. Pre-incubation of metacyclic trypomastigotes with scFv-10D8 showed a remarkable reduction in cell invasion capacity. Our results suggest that scFv-10D8 can be used in a paratransgenic approach to target parasites in insect vectors, avoiding dissemination of infective forms. Such advances in the development of this functional molecule will surely prompt the improvement of alternative strategies to control Chagas disease by targeting mammalian host stages.eng
dc.language.isoengeng
dc.publisherJavier Marcelo Di Noia, Institut de recherches cliniques de Montreal, CANADAeng
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourcePLoS ONEeng
dc.source14(10), (2019)spa
dc.source.urihttps://doi.org/10.1371/journal. pone.0223773eng
dc.subjectTrypanosoma cruzieng
dc.subjectParasitic diseaseseng
dc.subjectPeriplasmeng
dc.subjectChagas diseaseeng
dc.subjectProtein extractioneng
dc.subjectProtozoan infectionseng
dc.subjectSequence databaseseng
dc.subjectTrypomastigoteseng
dc.titleEngineering a single-chain antibody against Trypanosoma cruzi metacyclic trypomastigotes to block cell invasioneng
dc.typearticleeng
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
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