Profiling analysis of circulating microRNA in peripheral blood of patients with class IV lupus nephritis
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Fecha
2017-11-14
Autores
Navarro-Quiroz, Elkin
Pacheco-Lugo, Lisandro
Navarro-Quiroz, Roberto
Lorenzi, Hernan
España-Puccini, Pierine
DõÂaz-Olmos, Yirys
Almendrales, Lisneth
Olave, Valeria
Gonzalez-Torres, Henry
Diaz-Perez, Anderson
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Xu-jie Zhou, Peking University First
Hospital, CHINA
Resumen
Renal involvement in Systemic Lupus Erythematous (SLE) patients is one of the leading
causes of morbidity and a significant contributor to mortality. It's estimated that nearly 50% of
SLE individuals develop kidney disease in the first year of the diagnosis. Class IV lupus nephritis
(LN-IV) is the class of lupus nephritis most common in Colombian patients with SLE. Altered
miRNAs expression levels have been reported in human autoimmune diseases including
lupus. Variations in the expression pattern of peripheral blood circulating miRNAs specific for
this class of lupus nephritis could be correlated with the pathophysiological status of this group
of individuals. The aim of this study was to evaluate the relative abundance of circulating
microRNAs in peripheral blood from Colombian patients with LN-IV. Circulating miRNAs in
plasma of patients with diagnosis of LN-IV were compared with individuals without renal
involvement (LNN group) and healthy individuals (CTL group). Total RNA was extracted from
10 ml of venous blood and subsequently sequenced using Illumina. The sequences were processed
and these were analyzed using miRBase and Ensembl databases. Differential gene
expression analysis was carried out with edgeR and functional analysis were done with
DIANA-miRPath. Analysis was carried out using as variables of selection fold change ( 2 o
-2) and false discovery rate (0.05). We identified 24 circulating microRNAs with differential
abundance between LN-IV and CTL groups, fourteen of these microRNAs are described for
the first time to lupus nephritis (hsa-miR-589-3p, hsa-miR-1260b, hsa-miR-4511, hsa-miR-
485-5p, hsa-miR-584-5p, hsa-miR-543, hsa-miR-153-3p, hsa-miR-6087, hsa-miR-3942-5p,
hsa-miR-7977, hsa-miR-323b-3p, hsa-miR-4732-3p and hsa-miR-6741-3p). These changes
in the abundance of miRNAs could be interpreted as alterations in the miRNAs-mRNA regulatory
network in the pathogenesis of LN, preceding the clinical onset of the disease. The findings
thus contribute to understanding the disease process and are likely to pave the way
towards identifying disease biomarkers for early diagnosis of LN.
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Lupus