Evaluación de la capacidad que tienen los niveles de ácido desoxirribonucleico libre circulante mitocondrial y nuclear urinario para discriminar muestras de sujetos con nefritis lúpica
| datacite.rights | http://purl.org/coar/access_right/c_16ec | spa |
| dc.contributor.advisor | Navarro, Elkin | |
| dc.contributor.advisor | Navarro, Roberto | |
| dc.contributor.author | Márquez Escobar, Olga Cecilia | |
| dc.date.accessioned | 2022-08-01T16:07:18Z | |
| dc.date.available | 2022-08-01T16:07:18Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | El lupus eritematoso sistémico (LES), es una enfermedad autoinmune, proclive a una presentación clínica heterogénea que debuta de manera silente en casos de nefritis lúpica activa (NLA), lo cual es mandatorio de una biopsia renal percutánea con consecuentes eventos adversos. Objetivo: Evaluar la capacidad que tienen los niveles de ADN libre circulante mitocondrial y nuclear urinario (uADN-lc) para discriminar muestras de sujetos con nefritis lúpica activa (NLA). Sujetos y Métodos: Se tomaron muestras de orina de (74) sujetos, (25) con LES sin compromiso renal, (12) con NLA (12) con NLR y (25) Controles sanos. Se realizó una reacción en cadena de la polimerasa en tiempo real (qPCR) para la cuantificación de las moléculas de ácido desoxirribonucleico libre circulante (ADN-lc), mediante el empleo de genes constitutivos ND1 (mitocondrial) y GAPDH (nuclear), a posteriori de su extracción urinaria (uADN-lc), mediante el Kit Comercial de Aislamiento con Perlas Magnéticas (Applied Biosystems), y la determinación de su concentración con espectrofotómetro (Nanodrop One 2000 de Thermo Scientific ®) y analizador de imágenes. | spa |
| dc.description.abstract | Systemic lupus erythematous (SLE) is an autoimmune disease, prone to a heterogeneous clinical presentation that debuts silently in cases of active lupus nephritis (ALN), which is mandatory for a percutaneous renal biopsy with consequent adverse events. Objective: To evaluate the ability of mitochondrial and nuclear urinary cell-free DNA (cf-uDNA) levels to discriminate samples from subjects with active lupus nephritis (ALN). Subjects and Methods: Urine samples were taken from (74) subjects, (25) with SLE without kidney failure, (12) with (ALN), (12) with (RLN) and (25) Healthy Controls. A quantitative real-time polymerase chain reaction (Q-PCR) was performed for cell-free deoxyribonucleic acid molecule quantification (cf-DNA), using constitutive genes ND1 (mitochondrial) and GAPDH (nuclear), after their urinary extraction (cf-uDNA), by means of the Commercial Isolation Kit with Magnetic Pearls (Applied Biosystems), and its concentration measurement with spectrophotometer (Nanodrop One 2000 de Thermo Scientific ®) and image analyzer. | eng |
| dc.format.mimetype | spa | |
| dc.identifier.uri | https://hdl.handle.net/20.500.12442/10436 | |
| dc.language.iso | spa | spa |
| dc.publisher | Ediciones Universidad Simón Bolívar | spa |
| dc.publisher | Facultad de Ciencias Básicas y Biomédicas | spa |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | eng |
| dc.rights.accessrights | info:eu-repo/semantics/restrictedAccess | spa |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Complejos inmunes | spa |
| dc.subject | Concentración de ácido desoxirribonucleico libre circulante urinario (uADN-lc) | spa |
| dc.subject | GAPDH | spa |
| dc.subject | SLEDAI | spa |
| dc.subject | SLICC | spa |
| dc.subject | ND1 | spa |
| dc.subject | Nefritis lúpica activa (NLA) | spa |
| dc.subject | Concentration of urinary circulating free deoxyribonucleic acid (uDNA-lc) | eng |
| dc.subject | Active lupus nephritis (ANL) | eng |
| dc.title | Evaluación de la capacidad que tienen los niveles de ácido desoxirribonucleico libre circulante mitocondrial y nuclear urinario para discriminar muestras de sujetos con nefritis lúpica | spa |
| dc.title.translated | Assessment of the ability of mitochondrial and nuclear urinary cell free circulating deoxyribonucleic acid levels to discriminate samples from subjects with Lupus Nephritis | eng |
| dc.type.driver | info:eu-repo/semantics/masterThesis | spa |
| dc.type.spa | Trabajo de grado máster | spa |
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| oaire.version | info:eu-repo/semantics/acceptedVersion | spa |
| sb.programa | Maestría en Genética | spa |
| sb.sede | Sede Barranquilla | spa |