Validación y verificación de un protocolo in house con nanopartículas magnéticas para la extracción de ácidos nucleicos de SARS-CoV-2
| datacite.rights | http://purl.org/coar/access_right/c_f1cf | |
| dc.contributor.advisor | Acosta Hoyos, Antonio José | |
| dc.contributor.advisor | Pachecho Londoño, Leonardo | |
| dc.contributor.author | Flórez Rojas, Jullieth Katherinne | |
| dc.date.accessioned | 2025-02-06T22:09:28Z | |
| dc.date.available | 2025-02-06T22:09:28Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | La pandemia generada por COVID-19 causó crisis sanitaria a nivel mundial, en parte por el desabastecimiento de insumos médicos, entre ellos las pruebas diagnósticas de SARS-CoV-2 donde el problema se agudizó en países como Colombia y otros en América Latina. Los países en desarrollo donde la financiación en los sistemas de atención médica es limitada generó consecuencias que provocaron el aumento en la mortalidad y limitó la detección temprana del virus. En la búsqueda de herramientas importantes que permitieran abordar la problemática, favorecer la investigación biomédica básica y el diagnóstico clínico, se diseñó y optimizo un método de extracción de ácidos nucleicos in house el cual permita mejorar la accesibilidad y contribuir a la independencia biotecnológica en el contexto local. En el presente estudio se tuvo como objetivo presentar un método de síntesis por coprecipitación de nanopartículas magnéticas de hierro sin recubrimiento, basado en la síntesis de BOMB (1), diseñadas específicamente para la recuperación de material genético, garantizando tanto su eficiencia como su calidad. Para asegurar la viabilidad de este enfoque, se evaluó la robustez del método de síntesis y se estandarizó el proceso de creación de nanopartículas magnéticas; así mismo se evalúo la efectividad de las soluciones de lisis que hacen parte del kit de extracción de ácidos nucleicos. Además, se realizó una estimación de costos del kit in house con el fin de evaluar su viabilidad, resultando el kit in house un 93.7 % más económico en comparación con el precio del kit comercial | spa |
| dc.description.abstract | The pandemic generated by COVID-19 caused a worldwide health crisis, mainly due to the shortage of medical supplies, including diagnostic tests for SARS-CoV-2, and the problem worsened in countries such as Colombia and others in Latin America. In developing countries, funding in health care systems is limited, which has had consequences that have led to increased mortality and limited early detection of the virus. In the search for important tools to address the problem, promote basic biomedical research and clinical diagnosis, an in house nucleic acid extraction method was designed and optimized to improve accessibility and contribute to biotechnological independence in the local context. The present study aimed to present a method for the co-precipitation synthesis of uncoated magnetic iron nanoparticles based on BOMB synthesis (Oberacker et al., 2019), specifically designed for the recovery of genetic material, ensuring both efficiency and quality. To ensure the feasibility of this approach, the robustness of the synthesis method was evaluated and the process of creating magnetic nanoparticles was standardized, as well as the effectiveness of the lysis solutions that are part of the nucleic acid extraction kit was evaluated. In addition, a cost estimation of the in-house kit was made in order to evaluate its viability, obtaining that the in house kit was 93.7 % cheaper, compared to the price of the commercial kit, in addition the in house kit showed better performance in relation to allowing a greater number of tests to be performed | eng |
| dc.format.mimetype | ||
| dc.identifier.uri | https://hdl.handle.net/20.500.12442/16230 | |
| dc.language.iso | spa | |
| dc.publisher | Ediciones Universidad Simón Bolívar | spa |
| dc.publisher | Facultad de Ciencias Básicas y Biomédicas | spa |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | eng |
| dc.rights.accessrights | info:eu-repo/semantics/embargoedAccess | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | |
| dc.subject | Nanopartículas magnéticas | spa |
| dc.subject | Extracción de ácidos nucleicos | spa |
| dc.subject | Validación | spa |
| dc.subject.keywords | Magnetic nanoparticles | eng |
| dc.subject.keywords | Nucleic acid extraction | eng |
| dc.subject.keywords | Validation | eng |
| dc.title | Validación y verificación de un protocolo in house con nanopartículas magnéticas para la extracción de ácidos nucleicos de SARS-CoV-2 | spa |
| dc.type.driver | info:eu-repo/semantics/masterThesis | |
| dc.type.spa | Trabajo de grado máster | |
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| oaire.version | info:eu-repo/semantics/acceptedVersion | |
| sb.investigacion | Nuevos mMétodos diagnósticos | |
| sb.programa | Maestría en Genética | spa |
| sb.sede | Sede Barranquilla | spa |
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