Aislamiento e identificación de bacterias resistentes al mercurio en sedimentos de la ciénaga de Mallorquín, Atlántico, Colombia
datacite.rights | http://purl.org/coar/access_right/c_16ec | spa |
dc.contributor.advisor | Soto Varela, Zamira Elena | |
dc.contributor.advisor | Peña Freile, Mario | |
dc.contributor.author | Bravo Ortiz, Valentina | |
dc.contributor.author | Consuegra Padilla, Natalia Carolina | |
dc.contributor.author | Robledo Meza, Alfredo de Jesús | |
dc.date.accessioned | 2022-12-07T18:34:56Z | |
dc.date.available | 2022-12-07T18:34:56Z | |
dc.date.issued | 2022 | |
dc.description.abstract | La contaminación con metales pesados son una de las problemáticas ambientales que más afectan a los ecosistemas acuáticos, uno de los factores que influyen en esto son las prácticas industriales y mineras que arrojan los metales pesados como desechos a los afluentes y que pueden ocasionar la muerte de la flora, fauna, diversidad microbiana e incluso a los habitantes que rodean esta zona, causando afectaciones en órganos o tejidos e incluso cáncer. El objetivo de esta investigación es determinar la presencia de bacterias tolerantes al mercurio en muestras de sedimentos en la Ciénaga de Mallorquín ubicada en el Departamento del Atlántico, Colombia con potencial para una futura biorremediación de este ecosistema. Las muestras de sedimentos fueron tomadas de 4 puntos de la ciénaga, a partir de las cuales se realizaron cultivos para el aislamiento de bacterias. A los aislados bacterianos se les realizaron pruebas de susceptibilidad al mercurio en su forma de sal (HgCl) con concentraciones de 0,01 mM, 0,05 mM, 0,1 mM, 0,25 mM 0,50 mM y 0,75 mM. Las cepa 003 Micrococcus aloeverae y 008 Halobacillus dabanensis fueron las que tuvieron mayor tolerancia al crecer a concentraciones de 0,5 mM. Por último, el aislamiento de bacterias que resisten altas concentraciones de Hg abre la posibilidad en un futuro de biorremediar los ecosistemas contaminados con este metal pesado | spa |
dc.description.abstract | Heavy metals are one of the environmental problems that most affect lagoon ecosystems, this is caused by industrial and mining practices that throw heavy metals as waste into tributaries and that can cause the death of flora and fauna, causing damage to organs or tissues and even cancer. The objective of this research is to determine the presence of mercury-tolerant bacteria in sediment samples in the Majorcan swamp located in the Department of Atlántico, Colombia with potential for future bioremediation of this ecosystem. The sediment samples were taken from 4 points of the swamp, from which cultures were made for the isolation of bacteria; Bacterial isolates were tested for susceptibility to mercury in its salt form (HgCl) with concentrations of 0.01 mM, 0.05 mM, 0.1 mM, 0.25 mM, 0.50 mM and 0.75 mM. Strain 003 Micrococcus aloeverae and 008 Halobacillus dabanensis were the ones with the highest tolerance when growing at concentrations of 0.5 mM. Finally, the genetic identification of resistant bacteria was done using the 16S rRNA gene. The isolation of bacteria that resist high concentrations of Hg opens the possibility in the future of bioremediating ecosystems contaminated with this heavy metal. | eng |
dc.format.mimetype | spa | |
dc.identifier.uri | https://hdl.handle.net/20.500.12442/11664 | |
dc.language.iso | spa | spa |
dc.publisher | Ediciones Universidad Simón Bolívar | spa |
dc.publisher | Facultad de Ciencias Básicas y Biomédicas | spa |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.accessrights | info:eu-repo/semantics/restrictedAccess | spa |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Contaminación de agua | spa |
dc.subject | Laguna | spa |
dc.subject | Metales pesados | spa |
dc.subject | Resistencia bacteriana. | spa |
dc.subject | Bacterial resistance | eng |
dc.subject | Heavy metals | eng |
dc.subject | Lagoon | eng |
dc.subject | Pollutants | eng |
dc.subject | Water contamination | eng |
dc.title | Aislamiento e identificación de bacterias resistentes al mercurio en sedimentos de la ciénaga de Mallorquín, Atlántico, Colombia | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.spa | Trabajo de grado - pregrado | spa |
dcterms.references | Acevedo, R., & Severiche, C. (2013). Identification of di-bromide mercury Resistant Bacteria Isolated from Sediment Beaches in Cartagena de Indias, Colombian Caribbean. In AVANCES Investigación en Ingeniería (Vol. 10, Issue 2). https://revistas.unilibre.edu.co/index.php/avances/article/view/2750/2170 | eng |
dcterms.references | Basu, N., Horvat, M., Evers, D. C., Zastenskaya, I., Weihe, P., & Tempowski, J. (2018). A state-of-the-science review of mercury biomarkers in human populations worldwide between 2000 and 2018. In Environmental Health Perspectives (Vol. 126, Issue 10). Public Health Services, US Dept of Health and Human Services. https://doi.org/10.1289/EHP3904 | eng |
dcterms.references | Beltrán, M., & Gómez, A. (2016). Biorremediación de metales pesados cadmio (Cd), cromo (Cr) y mercurio (Hg), mecanismos bioquímicos e ingeniería genética: una revisión. Revista Facultad de Ciencias Básicas, 12(2), 172–197. https://doi.org/10.18359/rfcb.2027 | spa |
dcterms.references | Biedendieck, R., Knuuti, T., Moore, S. J., & Jahn, D. (n.d.). The “beauty in the beast”-the multiple uses of Priestia megaterium in biotechnology. https://doi.org/10.1007/s00253-021- 11424-6/Published | eng |
dcterms.references | Bolívar, D. (2020). CUANTIFICACIÓN DE METALES PESADOS (HG, CR, CD, NI) EN SEDIMENTOS DE LA CIÉNAGA MALLORQUÍN, BARRANQUILLA COLOMBIA. https://repositorio.cuc.edu.co/bitstream/handle/11323/7269/CUANTIFICACI%C3% 93N%2 0DE%20METALES%20PESADOS%20%28HG%2C%20CR%2C%20CD%2C%20NI%2 9 %20EN%20SEDIMENTOS%20DE%20LA%20CI%C3%89NAGA%20MALLORQU%C 3 %8DN%2C%20BARRANQUILLA%20- %20COLOMBIA.pdf?sequence=1&isAllowed=y | spa |
dcterms.references | Cardona, G. I., Escobar, M. C., Acosta-González, A., Marín, P., & Marqués, S. (2022). Highly mercury-resistant strains from different Colombian Amazon ecosystems affected by artisanal gold mining activities. Applied Microbiology and Biotechnology, 106(7), 2775– 2793. https://doi.org/10.1007/s00253-022-11860-y | eng |
dcterms.references | Fajardo Vidal, N., & Solís Acosta, H. (2017). Determination of heavy metals in water bodies from the Regional Conservation Area Wetlands of Ventanilla-Callao, Peru (Vol. 20). https://doi.org/10.15381/iigeo.v20i39.14177 | eng |
dcterms.references | García, L., Marrugo, J., & Alvis, E. (2010). Contaminación por mercurio en humanos y peces en el municipio de Ayapel, Córdoba, Colombia, 2009Mercury. 118–124. | spa |
dcterms.references | Giraldo, Jd., Gutiérrez, S., & Merino, F. (2014). OIL EMULSIFYING ACTIVITY AND REMOVAL OF HEAVY METALS BY PSEUDOMONAS AERUGINOSA PB 25 RHAMNOLIPID. In Rev Soc Quím Perú (Vol. 80, Issue 1). http://www.scielo.org.pe/scielo.php?script=sci_arttext&pid=S1810- 634X2014000100005#:~:text=El%20ramnol%C3%ADpido%20producido%20por %20P,e mpleo%20en%20procesos%20de%20biorremediaci%C3%B3n. | eng |
dcterms.references | Gómez, P., & Gloria de las M. (2005). Importancia económico-ambiental del ecosistema manglar. 138, 111–134. https://www.redalyc.org/pdf/4255/425541308005.pdf | spa |
dcterms.references | Gupta, R. S., Patel, S., Saini, N., & Chen, S. (2020). Robust demarcation of 17 distinct bacillus species clades, proposed as novel bacillaceae genera, by phylogenomics and comparative genomic analyses: Description of robertmurraya kyonggiensis sp. nov. and proposal for an emended genus bacillus limiting it only to the members of the subtilis and cereus clades of species. International Journal of Systematic and Evolutionary Microbiology, 70(11), 5753–5798. https://doi.org/10.1099/ijsem.0.004475 | eng |
dcterms.references | Guzmán-Moreno, J., García-Ortega, L. F., Torres-Saucedo, L., Rivas-Noriega, P., Ramírez- Santoyo, R. M., Sánchez-Calderón, L., Quiroz-Serrano, I. N., & VidalesRodríguez, L. E. (2022). Bacillus megaterium HgT21: a Promising Metal Multiresistant Plant Growth- Promoting Bacteria for Soil Biorestoration. Microbiology Spectrum. https://doi.org/10.1128/spectrum.00656-22 | eng |
dcterms.references | Invemar. (2020). Diagnóstico y evaluación de la calidad de las aguas marinas y costeras del caribe y pacífico colombianos. Instituto de Investigaciones Marinas y Costeras “José Benito de Andréis.”. http://cinto.invemar.org.co/alfresco/d/d/workspace/SpacesStore/bf9631aa- cd73- 48be-8ee4- 73251a1f7c03/Diagn%C3%B3stico%20y%20evaluaci%C3%B3n%20de%20la%20 calidad %20de%20las%20aguas%20marinas%20y%20costeras%20en%20el%20Caribe%2 0y%20P ac%C3%ADfico%20colombianos?ticket=TICKET_41500af697e9457eb0eb1630e36 0d141f 6ed5dfe | spa |
dcterms.references | Journal, I., Noroozi, M., Ali Amoozegar, M., Ali Pourbabaee, A., Naghavi, S., & Nourmohammadi, Z. (2017). Evaluation of the Moderately Halophilic Bacteria Resistant to Mercury from Saline Soil 1 2*. In Ecology (Vol. 44, Issue 2). | eng |
dcterms.references | Kheiralla, Z. H., Ashour, S. M., Rushdy, A. A., & Ahmed, H. A. (2013). Characterization of biosurfactants produced by Halobacillus dabanensis and Pontibacillus chungwhensi isolated from oil-contaminated mangrove ecosystem in Egypt. Applied Biochemistry and Microbiology, 49(3), 263–269. https://doi.org/10.1134/S0003683813030186 | eng |
dcterms.references | Lebeau, T., Bagot, D., Jezequel, K., & Fabre, B. (2002). Cadmium biosorption by free and immobilised microorganisms cultivated in a liquid soil extract medium: effects of Cd, pH and techniques of culture. In The Science of the Total Environment (Vol. 291). 10.1016/s0048- 9697(01)01093-2 | eng |
dcterms.references | Liu, W. Y., Zeng, J., Wang, L., Dou, Y. T., & Yang, S. S. (2005). Halobacillus dabanensis sp. nov: And Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang, China. International Journal of Systematic and Evolutionary Microbiology, 55(5), 1991–1996. https://doi.org/10.1099/ijs.0.63787-0 | eng |
dcterms.references | Londoño Franco, L. F., Londoño Muñoz, P. T., & Muñoz Garcia, F. G. (2016). LOS RIESGOS DE LOS METALES PESADOS EN LA SALUD HUMANA Y ANIMAL. Biotecnoloía En El Sector Agropecuario y Agroindustrial, 14(2), 145. https://doi.org/10.18684/bsaa(14)145-153 | spa |
dcterms.references | Massilamany, C., Mohammed, A., Loy, J. D., Purvis, T., Krishnan, B., Basavalingappa, R. H., Kelley, C. M., Guda, C., Barletta, R. G., Moriyama, E. N., Smith, T. P. L., & Reddy, J. (2016). Whole genomic sequence analysis of Bacillus infantis: Defining the genetic blueprint of strain NRRL B-14911, an emerging cardiopathogenic microbe. BMC Genomics, 17. https://doi.org/10.1186/s12864-0162900-2 | eng |
dcterms.references | Mesquidaz, E., Marrugo, J., & Pinedo, H. (2013). Exposición a mercurio en trabajadores de una mina de oro en el norte de Colombia. 29, 534–541. http://www.scielo.org.co/pdf/sun/v29n3/v29n3a13.pdf | spa |
dcterms.references | Michael, G. (2000). Bioremedial potential of microbial mechanisms of metalmobilization and immobilization. Environmental Biotechnology, 271–279. | eng |
dcterms.references | Muñoz, M., & Domínguez, Lady. (2013). IQEN INFORME QUINCENAL EPIDEMIOLÓGICO NACIONAL. https://www.ins.gov.co/buscador/IQEN/IQEN%20vol%2018%202013%20num% 204.pdf | spa |
dcterms.references | Navarro-Torre, S., Mateos-Naranjo, E., Caviedes, M. A., Pajuelo, E., & Rodríguez-Llorente, I. D. (2016). Isolation of plant-growth-promoting and metal-resistant cultivable bacteria from Arthrocnemum macrostachyum in the Odiel marshes with potential use in phytoremediation. Marine Pollution Bulletin, 110(1), 133–142. https://doi.org/10.1016/j.marpolbul.2016.06.070 | eng |
dcterms.references | Pabón, S., Benítez, R., Sarria, A., & Gallo, A. (2020). Contaminación del agua por metales pesados, métodos de análisis y tecnologías de remoción. Una revisión. Entre Ciencia e Ingeniería, 14, 9–18. https://doi.org/10.31908/19098367.1734 | spa |
dcterms.references | Paul, S. I., Rahman, M. M., Salam, M. A., Khan, M. A. R., & Islam, M. T. (2021). Identification of marine sponge-associated bacteria of the Saint Martin’s island of the Bay of Bengal emphasizing on the prevention of motile Aeromonas septicemia in Labeo rohita. Aquaculture, 545. https://doi.org/10.1016/j.aquaculture.2021.737156 | eng |
dcterms.references | Peter, A., Ford, S., & Brown, L. (1986). Transcriptional Regulation of the Mercuryresistance Genes of Transposon Tn501. In Journal of’ General Microbiology. 10.1099/00221287-132- 2-465 | eng |
dcterms.references | Portz, L., Manzolli, R. P., de Andrade, C. F. F., Villate Daza, D. A., Bolivar Bandeira, D. A., & Alcántara-Carrió, J. (2020). Assessment of Heavy Metals Pollution (Hg, Cr, Cd, Ni) in the Sediments of Mallorquin Lagoon - Barranquilla, Colombia. Journal of Coastal Research, 95(sp1), 158–162. https://doi.org/10.2112/SI95-031.1 28. Prakash, O., Nimonkar, Y., Munot, H., Sharma, A., Vemuluri, V. R. amana, Chavadar, M. S., & Shouche, Y. S. (2014). Description of Micrococcus aloeverae sp. nov., an endophytic actinobacterium isolated from Aloe vera. International Journal of Systematic and Evolutionary Microbiology, 64, 3427–3433. https://doi.org/10.1099/ijs.0.063339-0 | eng |
dcterms.references | Prieto, J., Gonzáles, R., Romá, G., & Prieto, F. (2009). CONTAMINACIÓN Y FITOTOXICIDAD EN PLANTAS POR METALES PESADOS PROVENIENTES DE SUELOS Y AGUA. Tropical and Subtropical Agroecosystems, 10(1), 29–44. http://www.redalyc.org/articulo.oa?id=93911243003 | eng |
dcterms.references | Priyadarshanee, M., Chatterjee, S., Rath, S., Dash, H. R., & Das, S. (2022). Cellular and genetic mechanism of bacterial mercury resistance and their role in biogeochemistry and bioremediation. In Journal of Hazardous Materials (Vol. 423). Elsevier B.V. https://doi.org/10.1016/j.jhazmat.2021.126985 | eng |
dcterms.references | Purkan, P., Nuzulla, Y. F., Hadi, S., & Prasetyawati, E. T. (2017). Biochemical Properties of Mercuric Reductase from Local Isolate of Bacillus sp for Bioremediation Agent. Molekul, 12(2), 182. https://doi.org/10.20884/1.jm.2017.12.2.398 | eng |
dcterms.references | Rajendran, P., Muthukrishnan, J., & Gunasekaran, P. (2003). Microbes in heavy metal remediation. Indian Journal of Experimental Biology, 41, 935–944. | eng |
dcterms.references | Reyes, Y. C., Vergara, I., Torres, O. E., Díaz, M., & González, E. E. (2016). Heavy metals contamination: implications for health and food safety. 16, 66–77. | eng |
dcterms.references | Vega Alonso, J. (2015). INTERACCIÓN DE PHOTOBACTERIUM DAMSELAE SUBSP. PISCICIDA CON CÉLULAS SAF-1. | spa |
dcterms.references | Zarza, L. (2017). Historias del agua (3): El desastre de Minamata. https://www.iagua.es/blogs/laura-f-zarza/historias-agua-3-desastre-minamata | spa |
oaire.version | info:eu-repo/semantics/acceptedVersion | spa |
sb.programa | Microbiología | spa |
sb.sede | Sede Barranquilla | spa |