Análisis de patrones genéticos comunes relacionados con la colonización de diversas bacterias endófitas aisladas de Rhizophora sp. y Avicennia sp.
Fecha
2024
Autores
Castañeda Herrera, Natalia Sofía
Prada Narváez, Laura Vanessa
Sandoval Guerra, Jersy Johanna
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Editor
Ediciones Universidad Simón Bolívar
Facultad de Ciencias Básicas y Biomédicas
Facultad de Ciencias Básicas y Biomédicas
Resumen
Los manglares, ubicados en zonas tropicales y subtropicales, son ecosistemas clave que brindan importantes servicios ecosistémicos, como la conservación de la biodiversidad y la captura de CO₂. En estos entornos, los microorganismos endófitos desempeñan un rol esencial al mejorar la tolerancia al estrés de las plantas y aportar nutrientes, favoreciendo así la estabilidad del ecosistema. Sin embargo, las investigaciones sobre los patrones genéticos que posibilitan esta simbiosis son limitadas.
Este estudio tuvo como objetivo identificar y caracterizar los patrones genéticos relacionados con la colonización de bacterias endófitas en Rhizophora sp y Avicennia sp. Mediante una revisión de la literatura científica y el análisis de genomas descargados del NCBI, se evaluó la calidad del genoma con QUAST. Para caracterizar los patrones genéticos, se realizaron búsquedas pBLAST de secuencias de proteínas relacionadas con los mecanismos de colonización de plantas; finalmente, se llevaron a cabo alineamientos en MEGA, generando árboles filogenéticos y analizando relaciones evolutivas basadas en genes 16S. El análisis de 28 especies bacterianas aisladas de Rhizophora sp (10) y Avicennia sp (18) mostró que los géneros predominantes fueron Bacillus y Staphylococcus, con Bacillus cereus como la especie más frecuentemente aislada. De las 36 secuencias de proteínas de referencia, 34 se encontraron en las bacterias endófitas, principalmente asociadas con la regulación de genes para la desintoxicación de especies reactivas de oxígeno (ROS) y la producción de enzimas líticas. Estos resultados indican que las bacterias endófitas de manglar poseen mecanismos de desintoxicación que les permiten adaptarse a condiciones adversas, facilitando su colonización en el tejido de plantas de manglar.
Mangroves, located in tropical and subtropical areas, are key ecosystems that provide important ecosystem services, such as biodiversity conservation and CO₂ capture. In these environments, endophytic microorganisms play an essential role in enhancing plant stress tolerance and supplying nutrients, thereby promoting ecosystem stability. However, research on the genetic patterns enabling this symbiosis is limited. This study aimed to identify and characterize the genetic patterns related to the colonization of endophytic microorganisms in Rhizophora and Avicennia. Through a review of scientific literature and the analysis of genomes downloaded from the NCBI, genome quality was evaluated using QUAST. To characterize genetic patterns, pblast searches were conducted on protein sequences associated with plant colonization mechanisms; finally, alignments were performed in MEGA, generating phylogenetic trees and analyzing evolutionary relationships based on 16S genes. The analysis of 28 bacterial species isolated from Rhizophora (10) and Avicennia (18) showed that the predominant genera were Bacillus and Staphylococcus, with Bacillus cereus being the most frequently isolated species. Of the 36 reference protein sequences, 34 were found in endophytic bacteria, mainly associated with gene regulation for the detoxification of reactive oxygen species (ROS) and the production of lytic enzymes. These findings indicate that mangrove endophytic bacteria possess detoxification mechanisms that allow them to adapt to adverse conditions, facilitating their colonization in mangrove plant tissues.
Mangroves, located in tropical and subtropical areas, are key ecosystems that provide important ecosystem services, such as biodiversity conservation and CO₂ capture. In these environments, endophytic microorganisms play an essential role in enhancing plant stress tolerance and supplying nutrients, thereby promoting ecosystem stability. However, research on the genetic patterns enabling this symbiosis is limited. This study aimed to identify and characterize the genetic patterns related to the colonization of endophytic microorganisms in Rhizophora and Avicennia. Through a review of scientific literature and the analysis of genomes downloaded from the NCBI, genome quality was evaluated using QUAST. To characterize genetic patterns, pblast searches were conducted on protein sequences associated with plant colonization mechanisms; finally, alignments were performed in MEGA, generating phylogenetic trees and analyzing evolutionary relationships based on 16S genes. The analysis of 28 bacterial species isolated from Rhizophora (10) and Avicennia (18) showed that the predominant genera were Bacillus and Staphylococcus, with Bacillus cereus being the most frequently isolated species. Of the 36 reference protein sequences, 34 were found in endophytic bacteria, mainly associated with gene regulation for the detoxification of reactive oxygen species (ROS) and the production of lytic enzymes. These findings indicate that mangrove endophytic bacteria possess detoxification mechanisms that allow them to adapt to adverse conditions, facilitating their colonization in mangrove plant tissues.
Descripción
Palabras clave
Manglares, Bacterias, Endófitos, Rhizophora, Avicennia