Examinando por Autor "Acosta-Hoyos, Antonio"
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Ítem DNA repair and metabolic gene polymorphisms affect genetic damage due to diesel engine exhaust exposure(Springer, 2020-03) León-Mejía, Grethel; Quintana-Sosa, Milton; de Moya Hernandez, Yurina; Luna Rodríguez, Ibeth; Trindade, Cristiano; Anaya Romero, Marco; Luna-Carrasca, Jaime; Oliveros Ortíz, Ludis; Acosta-Hoyos, Antonio; Ruiz-Benitez, Martha; Franco Valencia, Karen; Rohr, Paula; da Silva, Juliana; Pêgas Henriques, João AntônioDiesel engine exhaust (DEE) is a complex mixture of toxic gases, halogenated aromatic hydrocarbons, alkyl polycyclic aromatic hydrocarbons, polycyclic aromatic hydrocarbons, benzene derivatives, metals and diesel exhaust particles (DEPs) generated from the incomplete combustion of diesel fuel. Many of the compounds in this mixture can cause oxidative damage to DNA and are considered carcinogenic for humans. Further, chronic DEE exposure increases risks of cardiovascular and pulmonary diseases. Despite these pervasive health risks, there is limited and inconsistent information regarding genetic factors conferring susceptibility or resistance to DEE genotoxicity. The present study evaluated the effects of polymorphisms in two base excision repair (BER) genes (OGG1 Ser326Cys and XRCC1 Arg280His), one homologous recombination (HRR) gene (XRCC3 Thr241Met) and two xenobiotic metabolism genes (GSTM1 and GSTT1) on the genotoxicity profiles among 123 mechanics exposed to workplace DEE. Polymorphisms were determined by PCR-RFLP. In comet assay, individuals with the GSTT1 null genotype demonstrated significantly greater % tail DNA in lymphocytes than those with non-null genotype. In contrast, these null individuals exhibited significantly lower frequencies of binucleated (BN) cells and nuclear buds (NBUDs) in buccal cells than non-null individuals. Heterozygous hOGG1 326 individuals (hOGG1 326 Ser/Cys) exhibited higher buccal cell NBUD frequency than hOGG1 326 Ser/Ser individuals. Individuals carrying the XRCC3 241 Met/Met polymorphism also showed significantly higher buccal cell NBUD frequencies than those carrying the XRCC3 241 Thr/Thr polymorphism. We found a high flow of particulate matter with a diameter of < 2.5 μm (PM2.5) in the workplace. The most abundant metals in DEPs were iron, copper, silicon and manganese as detected by transmission electron microscopy–energy-dispersive X-ray spectroscopy (TEM-EDX). Scanning electron microscopy (SEM-EDS) revealed particles with diameters smaller than PM2.5, including nanoparticles forming aggregates and agglomerates. Our results demonstrate the genotoxic effects of DEE and the critical influence of genetic susceptibility conferred by DNA repair and metabolic gene polymorphisms that shed light into the understanding of underlying mechanisms.Ítem Genetic variation underpinning ADHD risk in a caribbean community(Published by MDP, 2019) Puentes-Rozo, Pedro J.; Acosta-López, Johan E.; Cervantes-Henríquez, Martha L.; Martínez-Banfi, Martha L.; Mejia-Segura, Elsy; Sánchez-Rojas, Manuel; Anaya-Romero, Marco E.; Acosta-Hoyos, Antonio; García-Llinás, Guisselle A.; Mastronardi, Claudio A.; Pineda, David A.; Castellanos, F. Xavier; Arcos-Burgos, Mauricio; Vélez, Jorge I.Attention Deficit Hyperactivity Disorder (ADHD) is a highly heritable and prevalent neurodevelopmental disorder that frequently persists into adulthood. Strong evidence from genetic studies indicates that single nucleotide polymorphisms (SNPs) harboured in the ADGRL3 (LPHN3), SNAP25, FGF1, DRD4, and SLC6A2 genes are associated with ADHD. We genotyped 26 SNPs harboured in genes previously reported to be associated with ADHD and evaluated their potential association in 386 individuals belonging to 113 nuclear families from a Caribbean community in Barranquilla, Colombia, using family-based association tests. SNPs rs362990-SNAP25 (T allele; p = 2.46 10x-4), rs2282794-FGF1 (A allele; p = 1.33 10x-2), rs2122642-ADGRL3 (C allele, p = 3.5 10x-2), and ADGRL3 haplotype CCC (markers rs1565902-rs10001410-rs2122642, OR = 1.74, Ppermuted = 0.021) were significantly associated with ADHD. Our results confirm the susceptibility to ADHD conferred by SNAP25, FGF1, and ADGRL3 variants in a community with a significant African American component, and provide evidence supporting the existence of specific patterns of genetic stratification underpinning the susceptibility to ADHD. Knowledge of population genetics is crucial to define risk and predict susceptibility to disease.Ítem Recomendaciones y actualizaciones en atención para el manejo de pacientes VIH positivo(Ediciones Universidad Simón Bolívar, 2018-07-01) Arquez Mendoza, Moises; Fontalvo Muñoz, Martha; Heredia Jiménez, Laura; Montaño Jiménez, Alejandra; Luna Carrascal, Jaime; Quintana Sosa, Milton; Acosta-Hoyos, AntonioEs un resumen de las guías de países desarrollados con la guía colombiana donde se observan varias diferencias importantes en el manejo de pacientes con VIH como se puede evidenciar en el desarrollo de la misma.Ítem VIH/SIDA Mitos y Verdades(Ediciones Universidad Simón Bolívar, 2018-07-01) Árquez Mendoza, Moisés; Luna Carrascal, Jaime; Quintana Sosa, Milton; Acosta-Hoyos, AntonioEl VIH, continúa siendo uno de los mayores problemas para la salud pública mundial, ha cobrado más de 35 millones de vidas, un millón de personas fallecieron en el mundo por causas relacionadas con este virus. Esta cartilla busca aclarar los mitos y verdades sobre el virus del VIH